Jan 06, 2017 · The cohesin complex (Smc1, Smc3, Scc1 and Scc3) safeguards genome integrity by ensuring correct sister-chromatid segregation during mitosis and meiosis 1,2,3.The topological entrapment of chromosomes by cohesin is catalysed by the cohesin–loader complex Scc2–Scc4 (refs 4, 5).In yeast, Scc2–Scc4 coordinates with the remodels structure of chromatin (RSC) chromatin …
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Jan 01, 2015 · Scc2 could regulate gene expression in many other ways such as serving as a "chromatin adaptor" for recruitment of other factors that maintain a nucleosome free region as recently reported. 7 Despite the tremendous work done in examining cohesin's role in gene expression, the molecular mechanisms are yet to be elucidated. More work is still required to fully understand the etiology of …
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Jul 15, 2015 · The SMC Loader Scc2 Promotes ncRNA Biogenesis and Translational Fidelity. Abstract. The Scc2-Scc4 we examined how the cohesin loader Scc2 regulates gene expression in bud-
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Oct 11, 2016 · III. Lindgren E., Hägg S., GIORDANO F., Björkegren J., and Ström L. Inactivation of the budding yeast cohesin loader Scc2 alters gene expression both globally and in response to a single DNA double strand break. Cell cycle, 2014, 12, 3645-58. Fulltext (DOI)
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Article DNA Binding by the Mis4 Scc2 Loader Promotes Topological DNA Entrapment by the Cohesin Ring Yumiko Kurokawa1 and Yasuto Murayama1,2,3,* 1Center for Frontier Research, National Institute of Genetics, 1111, Yata, Mishima, Shizuoka 411-8540, Japan 2Department of Genetics, SOKENDAI (The Graduate University for Advanced Studies), 1111, Yata, Mishima, Shizuoka 411-8540, Japan
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Mar 06, 2015 · Cohesin is also important for chromosome condensation, DNA repair, and gene expression. Cohesin is comprised of Scc3, Mcd1, Smc1, and Smc3. Scc3 also binds Pds5 and Wpl1, cohesin-associated proteins that regulate cohesin function, and to the Scc2/4 cohesin loader. We mutagenized SCC3 to elucidate its role in cohesin function. A 5 amino acid
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Hundreds of genes are differentially expressed in the scc2-4 mutant compared to WT at 30°C in YPD. (A) Gene expression values (mean of triplicate samples) are shown in MA plot.
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Apr 20, 2011 · Scc2 localizes to meiotic chromosomes, and its binding sites highly overlap with the cohesin-associated regions. To investigate chromosome association of Scc2 during yeast meiosis, we localized Scc2 and Rec8 on surface-spread nuclei by indirect immunofluorescence ().At pachytene, where homologues are paired and synapsed, Scc2, like Rec8, was localized along the length of the …
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Inactivation of the budding yeast cohesin loader Scc2 alters gene expression both globally and in response to a single DNA double strand break. Cell cycle, 2014, 12, 3645 -58. IV. Visnes T., GIOR DANO F., Kuznetsova A., Suja J. A., Lander A., Anne L Calof and Lena Str …
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Cohesin is a crucial component of chromosomal stability (Guacci et al., 1997; Losada et al., 1998; Michaelis et al., 1997).It consists of at least six subunits, the structural maintenance of chromosome (Smc) proteins Smc1 and Smc3, sister chromatid cohesion protein 1 (Scc1) (also known as Mcd1 or Rad21), Scc3 (also known as SA or stromalin), Pds5 and Wapl, which are all evolutionarily conserved.
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Jan 06, 2017 · The SMC Loader Scc2 Promotes ncRNA Biogenesis and Translational Fidelity. PLoS Genet. 11, e1005308 (2015). Article PubMed PubMed Central Google Scholar
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The SMC Loader Scc2 Promotes ncRNA Biogenesis and Translational Fidelity. The Scc2-Scc4 complex is essential for loading the cohesin complex onto DNA. Cohesin has important roles in chromosome segregation, DSB repair, and chromosome condensation. Here we report that Scc2 is important for gene expression in budding yeast.
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Furthermore, the Mis4 Scc2 loader was found to form a tertiary complex with cohesin on DNA, where its DNA binding activity facilitates the initial DNA binding of cohesin. In fission yeast cells, residues that support Mis4 Scc2 -DNA interaction are important for chromosomal cohesin loading and for cell viability.
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Jul 15, 2015 · Mutation of SCC2 was associated with defects in the production of ribosomal RNA, ribosome assembly, and splicing. While the scc2 mutant does not have a general defect in protein synthesis, it shows increased frameshifting and reduced cap-independent translation. These findings suggest Scc2 normally promotes a gene expression program that
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Jan 01, 2015 · Europe PMC is an archive of life sciences journal literature. This is a comment on "Inactivation of the budding yeast cohesin loader Scc2 alters gene expression both globally and in response to a single DNA double strand break." Cell Cycle. 2014;13(23):3645-58.
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Jan 01, 2014 · Here we extend these studies by investigating whether the cohesin loader Scc2 is involved in regulation of gene expression. We performed global gene expression profiling in the absence and presence of DNA damage, in wild type and Scc2 deficient G2/M arrested cells, when it is known that Scc2 is important for DNA double strand break repair and formation of damage induced cohesion.
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May 23, 2017 · In eukaryotic organisms, the correct regulation of sister chromatid cohesion, whereby sister chromatids are paired and held together, is essential for accurate segregation of the sister chromatids and homologous chromosomes into daughter cells during mitosis and meiosis, respectively. Sister chromatid cohesion requires a cohesin complex comprised of structural maintenance of …
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Jun 19, 2017 · Cohesin is a conserved, ring-shaped protein complex that encircles sister chromatids and ensures correct chromosome segregation during mitosis and meiosis. It also plays a crucial role in the regulation of gene expression, DNA condensation, and DNA repair through both non-homologous end joining and homologous recombination. Cohesins are spatiotemporally regulated by the Scc2–Scc4 …
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The sister chromatid cohesion apparatus mediates physical pairing of duplicated chromosomes. This pairing is essential for appropriate distribution of chromosomes into the daughter cells upon cell division. Recent evidence shows that the cohesion apparatus, which is a significant structural
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Furthermore, the Mis4 Scc2 loader was found to form a tertiary complex with cohesin on DNA, where its DNA binding activity facilitates the initial DNA binding of cohesin. In fission yeast cells, residues that support Mis4 Scc2 -DNA interaction are important for chromosomal cohesin loading and for cell viability.
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